Molecular devices corporation reserv es the right to change its products and services at any time to incorporate technological developments. Mitochondrial toxicity, spectramax minimax 300 cytometer. Molecular devices announce first microplate reader in space. Using the molecular devices test plate to calibrate the spectramax reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96 or 384well microplate. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular devices corporation reserves the right to change its products and services at any time to incorporate technological developments. Compoundinduced changes in observed beating rate can be correlated to cell viability for a more. The spectramax gemini xs uses two holographic diffraction grating monochromators which allow for individual optimization of wavelengths for both excitation and emission. Catchpoint simplestep elisa spectramax i3 i3x cathy olsen 20180917 23.
Fluorescence polarization spectramax detection cartridge fluorescence polarization spectramax detection cartridges offers great sensitivity for spectramax i3 and paradigm systems. Cardiomyocyte beating on spectramax i3, minimax imaging. Spectramax m3, m4, m5, and m5e multimode microplate. The format of an input file or stream may be associated with a oemolstream using the setformat method, and may be retrieved with getformat. The analyst, flexstation and spectramax m5m5 e from molecular devices have received the lanthascreen certified designation from life technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Spectramax i3 multimode detection platform introduction expression of fluorescent proteins, for example, green fluorescent proteins gfp, is often used as a readout for reporter gene assays. Automating scanlater western blot detection with stakmax. Scanlater western blot detection system molecular devices.
The spectramax i3x multimode microplate reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Spectramax i3x fl1 validation protocol evelyn mcgown 20151012 20. Researchers have created a variety of interesting molecular devices, including resonanttunneling diode rtd devices 4, 5, programmable molecular switches 6, carbon nanotube transistors, 7 and diodes 8, to name but a few. Microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Redox status in human erythrocytes and plasma was assayed using epr spectroscopy bruker xband epr spectrometer, emxmicro and colorimetric assays using spectramax i3 molecular devices, lls, usa. Fluorophore list 2 on the desktop of the computer a pdf file if you have two fluorophores, you may have better separation of the two signals by using nonpeak wavelengths. Investigate every aspect of a cellular pathway from imaging of cell confluence and viability under different treatment conditions to quantitation of nucleic acids and protein to western blot analysis, a wealth of new knowledge is. Genepix pro software is a tool mainly used for biological experiments, and pieces of data gathered from these experiments are saved in files with the. The spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of. Arcturusxt microdissection instrument user guide 011209 rev. Softmax pro data acquisition and analysis software. Monitoring cytotoxicity on spectramax i3, minimax cytometer molecular devices author. Softmax pro 7 data acquisition and analysis software. Spectramax i3 spectratest fl1 protocol evelyn mcgown 20726 16.
This user guide is subject to change without notice. Molecular devices and machines article pdf available in physics world 1711. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The spectramax i3x platform from molecular devices is a multimode microplate reader system that evolves with your future needs and offers an unlimited breadth of application possibilities. Spectramax m3 microplate reader from molecular devices llc. Fluorescence imaging was conducted on an fv 1200ix83 confocal laser scanning microscope olympus. Superior flexibility the spectramax i3 multimode platform comes with standard spectral absorbance, fluorescence, and luminescence detection.
This capabilities provide a tremendous new opportunity for. Creating interactive 3d pdf documents with vmd and acrobat3d. If the plate is new, or has been recertified since the last calibration, the new absorbance values must. Spectramax i3 spectratest lm1 protocol evelyn mcgown 20726 16. The spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities.
Molecular devices cleanable reader assy cleanable reader assy. Spectramax minimax 300 imaging cytometer molecular. Molecular devices 03102040 threshold cleanable reader only. Let molecular devices and the spectramax i3 x multimode detection platform power your next landmark discovery. In vivo fluorescence imaging was made on a kodak invivo imaging system fx pro. The two different data import options are tailored to quickly get your data into softmax pro to provide a single source of software for all your data reduction needs. The trademarks mentioned herein are the property of molecular devices, llc or their. The spectramax i3x multimode microplate reader measures spectralbased absorbance. The trademarks used herein are the property of molecular devices, llc or their respective owners. A arcturusxt microdissection instrument user guide molecular devices corporation 11 orleans drive sunnyvale, california 94089.
Hardware user guides and then click the document you want to view. This feature rich reader has a large, high resolution touchscreen with an. Spectramax gemini xs make it appropriate for applications within the. The absorbance at 490 nm was detected by spectramax i3 molecular devices, san jose, ca. Spectramax m3 multimode microplate reader, molecular devices. Molecular devices offers a wideranging portfolio of bioanalytical products and systems for life science research bioresearch. Htrf assays that utilize the tb donor with red d2 acceptor. Software for the test plate must be installed on the control computer before the plate can be used. Using the molecular devices test plate to calibrate the. Molecular devices corporation instrument warranty molecular devices corporation warrants this product against defects in material or workmanship as follows.
Spectramax m2m2e user guide university of michigan. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Cardiomyocyte beating i3 basic softmax pro protocol. Spectratest lm1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Molecular devices spectramax 250 microplate reader.
Setup molecular devices spectramax i3x tb green pdf. Conjugatedoligomers the term conjugated means an alternation of multiple and single bonds linking a sequence of bonded atoms, such that there is an extended series of overlapping. Molecular devices spectramax id3 multimode microplate reader. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. An ipone tb standard curve was run to assess performance of the spectramax i3 platform for. Script setup in the operations tab of softmax pro software, plate stacker is selected. Spectramax i3x fl1 validation protocol softmax pro.
Supporting information elegant molecular iodineantisolvent. Table 3 compatible fluorescence plate reader by manufacturer molecular devices bmg labtech. New applications in minutes adding modes and functionality is just minutes away. Molecular devices is one of the leading provider of highperformance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. The latest versions of adobe acrobat reader can now display, rotate, and animate 3d models embedded within these pdf documents.
Check your email, your application note is on the way. Reactions were quenched with phosphoric acid, bound to 96well p81 phosphocellulose filter plates millipore and assayed with microscint 20 scintillation fluid perkin elmer using a spectramax l luminometer molecular devices. Sunnyvale, ca prweb molecular devices announced today the launch of the new spectramax id3 multimode microplate reader, a cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Cardiomyocyte beating i3 basic cathy olsen 20726 19. With the spectramax minimax 300 imaging cytometer, industryleading softmax pro software, and user. The dual photomultiplier tube design on spectramax paradigm accelerates the throughput 2x due to the direct excitation optical path. Your total solution matched reagents, an imaging cytometer option, userupgradable. Multimode microplate reader spectramax i3, molecular devices. Softmax pro software user guide marine biological laboratory. Bioglo reagent promega corporation and quantitated with a multimode reader molecular devices.
Species made of interlocked molecular components are most attractive candidates for these purposes. Spectramax i3 multimode platform molecular devices. A new class of small molecule inhibitor of bmp signaling. The m5e reader includes industryleading softmax pro data analysis software that can meet all throughput, content, and multiplexing needs of researchers who conduct. Catchpoint simplestep elisa spectramax i3i3x softmax pro.
Imaging of histological sections was conducted on a bx43 fluorescence microscope olympus. Technical note in the stakmax window that opens, select scripting and choose file path new to create a new script. After assessing parallelism of the doseresponse curves, the potency of test sample relative to the reference standard was determined using graphpad prism graphpad software with the 4 parameter logistic model. Spectramax microplate spectrophotometer operators manual. The cell viability rate vr was calculated from the following equation. The percentage of cell viability was calculated by dividing the absorbance of the test group by the absorbance of the control group. Prepare data in the provided easytouse excel import templates import data into softmax pro from any non molecular devices microplate. The molecular devices spectramax 250 microplate reader has an optical design that mimics a dualbeam spectrophotometer.
Spectramax minimax 300 imaging cytometer molecular devices. Spectramax i3x multi mode detection platform molecular devices. Spectramax m3, m4, m5, and m5e multimode microplate reader. System enables firstofitskind western blot membrane detection in. When connecting to the spectramax i3x and i3 multimode detection. For a higher degree of automation, the automation vendor partners program has streamlined the integration of our microplate reader systems with all leading partner robots. Fluorescence, and luminescence with the added functionality of. Molecular devices and machines a journey into the nano. Spectratest fl1 this experiment contains the introduction this section, the certificate of calibration information certinfo, the. Molecular devices software programs and related file extensions.
Simply insert a cartridge to expand your application capabilities. Benralizumab for pdgfranegative hypereosinophilic syndrome. Fluorescence intensity spectramax paradigm or i3 detection. This is implemented in the formula system using the peakproanalysis operator, which can calculate sixteen different. Spectramax i3 spectratest fl1 protocol softmax pro. This document is in pdf format and can be viewed using a pdf viewer. Images were analyzed using molecular devices softmax pro 6. Spectratest instrument validation packages molecular devices. Nov 01, 2019 the organs were positioned on a lowfluorescence mat that was placed on a 96well plate insert of molecular devices i3 plate reader with a trf module wb cartridge and the plate was imaged using excitation of 340 nm and emission 615 nm.
Molecular devices topoftheline m5e reader offers flexible configurations across all commonlyused detection methods to accommodate a wide range of life sciences applications. We also discuss the different approaches that are used. Spectramax i3 spectratest lm1 protocol softmax pro. The energy needed to make a device work can be supplied as chemical energy, electrical energy, or light. This fieldupgradeable option with oneofakind brightfield cell segmentation, green.
The molecular mechanism of ginsenoside analogs activating. Unparalleled performance on a personalized platform. Clinical and biochemical data of endothelial function in. Following an introduction to the general concepts, the authors go on to discuss devices for processing electrons and electronic energy, memories, logic gates and related systems, and, finally, molecular. Ten years ago, chemical applications were limited to the use of small molecules such as. Spectramax m3, m4, m5, and m5 e multimode microplate. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Each sample has a discrete sample and reference beam so that each well is measured directly. Molecular devices introduces spectramax id3 multimode. Spectramax i3x multimode microplate reader molecular devices. Molecular devices spectramax id3 multimode microplate. Gfp transfection with spectramax i3 multimode detection.
A custom script was written to handle data file formatting, plate handling, data generation, and data storage. Molecular devices need energy to operate and signals to communicate with the operator. Plate reader instructions biomolecular analysis facility. Setup your microplate reader for optimal htrf readouts get on the list to receive remarkable content. Nanoantagonist alleviates inflammation and allows for mri of. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. After assessing parallelism of the doseresponse curves, s5 the sample binding relative to the reference standard was determined using graphpad prism graphpad software, san diego, california, usa with the 4. Microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices cor. Molecules have not historically played a prominent role in electronic devices.
The popular adobe pdf document format pdf version 1. Enabling the spectramax i3i3x and paradigm microplate readers antibodies designed to. In a typical reporter assay, the regulatory sequence of a gene of interest is attached to a reporter gene that can be easily detected and measured. Spectratest fl1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Explore how the spectramax minimax 300 imaging cytometer on the spectramax i3 multimode detection platform can be used to assess mitochondrial toxicity. We aim to give an impression of some of the different molecular electronic components and discuss the importance of molecular wires that should serve as interconnects between these devices. Molecular devices and machines the bottomup design, construction, and operation of devices and machines on the molecular scale is a topic of great interest in nanoscience and a fascinating challenge for nanotechnology. Prepare data in the provided easytouse excel import templates import data into softmax pro from any nonmolecular devices microplate.
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